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Projects

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ALE for yeast tolerance to lignocellulosic hydrolysates

(Saccharomyces cerevisiae)



New advances in adaptive evolution protocols, QTL mapping, and CRISPR/Cas9 technologies are proposed to enhance yeast tolerance to lignocellulosic hydrolysates. Learn more...

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EasyGuide CRISPR  ​(S. cerevisiae and E. coli)

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Leveraging in vivo cloning in yeast and E. coli to assemble gRNA and donor cassettes for multilocus edits, CRISPRi, CRISPRa, and base editors. EasyAssembler: rapid in vivo assembly of multipart DNA.

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ReMaSSing - Reiterated Mass Selection and backcrosSing (S. cerevisiae)

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A versatile platform for strain development that supports guided breeding, QTL mapping, genome debugging, and adaptive genotype selection.

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Adaptive evolution of Escherichia coli for  sucrose consumption

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We also conduct evolutionary experiments with E. coli, which we intend to use as a workhorse for the production of polyhydroxyalkanoates from sugarcane 

sucrose.

Experimental evolution of ethanol tolerance

(Saccharomyces cerevisiae PE-2)



Here we use the S. cerevisiae strain PE-2, widely used in the Brazilian bioethanol industry, to challenge cellular adaptation to an increasing ethanol concentration.

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Yeast experimental sexual evolution

(Schizosaccharomyces japonicus)



Here we use fission yeast to approach one of the most important questions in evolutionary biology: what are the advantages of sex over clonal propagation?

Learn more in our dedicated web page...

Genome Engineering and Evolution of Microorganisms

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