top of page

Modern Genetic Approaches to Boost Yeast Tolerance to Lignocellulosic Hydrolysates (LCH)

 

 

  • Novel experimental evolution protocols                                                                                   

  • New approaches to Quantitative Trait Loci (QTL) mapping                                       

  • Facile CRISPR/Cas9 technology to support genome editing                                     

  • Synthetic biology to redesign yeast cell to tolerate LCH and ferment xylose                                                                                                          

  • Flow Cytometry phenotyping of tolerant strains                                         

  • A robust collaborative network between key research groups

endrews0014_edited.jpg

Saccharomyces cerevisiae PE-2

 

Young Investigators grant 2017/24453-5

 

 

PROJECT SUMMARY 

We engineer Saccharomyces cerevisiae for 2G ethanol by improving tolerance to inhibitors in sugarcane lignocellulosic hydrolysates (LCHs). Using adaptive laboratory evolution, QTL mapping, next-generation sequencing, and flow-cytometry assays, we identify and validate the genetic bases of robustness. With the EasyGuide CRISPR/Cas9 platform and in vivo assembly, we build hyper-tolerant yeast chassis ready to receive additional traits—such as pentose metabolism—for high-performance cellulosic ethanol production.

RELATED PROJECTS

foto Ana.jpg

Dr. Ana P. Jacobus

Young Investigators awardee

WhatsApp Image 2020-09-02 at 19.12.34.jp

Yasmine A. Menegon

Ph.D. scholarship

Foto2.jpg

Lucas de Bem

M.Sc. scholarship

s008278-bp70-attune.jpg

Flow Cytometry

Facility

DSCN0248.JPG

Stella D. Cavassana

Undergraduate

scholarship

Isabelle I. Oliveira

Undergraduate

scholarship

Genome Engineering and Evolution of Microorganisms

  • w-googleplus
  • w-facebook
  • Twitter Clean
bottom of page