

Modern genetic approaches to bust yeast tolerance to lignocellulosic hydrolysates
(Saccharomyces cerevisae)
New advances in adaptive evolution protocols, QTL mapping, and CRISPR/Cas9 technologies are proposed to enhance yeast tolerance to lignocellulosic hydrolysates. Learn more...
Here we intend to engineer S. cerevisiae for the production of D-Lactic acid, a promising renewable material for production of bio-friendly plastics. Learn more...

GEvoLab - Genome Engineering and Evolution of Microorganisms
Using Synthetic Biology and Evolution to Design Microbial Genomes
Modern Genetic Approaches to Boost Yeast Tolerance to Lignocellulosic Hydrolysates (LCH)
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Novel experimental evolution protocols
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New approaches to Quantitative Trait Loci (QTL) mapping
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Facile CRISPR/Cas9 technology to support genome editing
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Synthetic biology to redesign yeast cell to tolerate LCH and ferment xylose
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Flow Cytometry phenotyping of tolerant strains
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A robust collaborative network between key research groups
Saccharomyces cerevisiae PE-2
Young Investigators grant 2017/24453-5
PROJECT SUMMARY
We engineer Saccharomyces cerevisiae for 2G ethanol by improving tolerance to inhibitors in sugarcane lignocellulosic hydrolysates (LCHs). Using adaptive laboratory evolution, QTL mapping, next-generation sequencing, and flow-cytometry assays, we identify and validate the genetic bases of robustness. With the EasyGuide CRISPR/Cas9 platform and in vivo assembly, we build hyper-tolerant yeast chassis ready to receive additional traits—such as pentose metabolism—for high-performance cellulosic ethanol production.
RELATED PROJECTS

Dr. Ana P. Jacobus
Young Investigators awardee

Yasmine A. Menegon
Ph.D. scholarship

Lucas de Bem
M.Sc. scholarship

Flow Cytometry
Facility

Stella D. Cavassana
Undergraduate
scholarship

Isabelle I. Oliveira
Undergraduate
scholarship