• Adaptive Laboratory Evolution (ALE) for stress tolerance in Saccharomyces cerevisiae and other microorganisms, uncovering genetic bases of robustness for industrial environments.                            

• Genome sequencing & bioinformatics to assemble genomes, map variants, interpret multi-omics data, and guide data-driven strain improvement.                                                                                        

• Genome engineering with the EasyGuide CRISPR suite and in vivo multipart DNA assembly, enabling precise, rapid, and scalable editing of microbial genomes.                                                              

• Design of microbial cell factories for bio-based products, translating synthetic biology and evolution into sustainable bioprocesses and next-generation biorefineries.

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We apply genomics and experimental evolution to Saccharomyces cerevisiae, Escherichia coli, and other microbes to uncover the genetic changes that drive adaptation to industrial stresses. Using the EasyGuide CRISPR toolkit and in vivo multipart DNA assembly, we perform precise, rapid genome edits to test genotype–phenotype relationships. Our aim is to understand evolution at the molecular level and to build robust microbial cell factories for sustainable, bio-based products. Our lab is located in Rio Claro at the Bioenergy Research Institute (IPBEN) of São Paulo State University (UNESP).