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  • Adaptive Laboratory Evolution (ALE) for stress tolerance in Saccharomyces cerevisiae and other microorganisms, uncovering genetic bases of robustness for industrial environments.                            

  • Genome sequencing & bioinformatics to assemble genomes, map variants, interpret multi-omics data, and guide data-driven strain improvement.                                                                                        

  • Genome engineering with the EasyGuide CRISPR suite and in vivo multipart DNA assembly, enabling precise, rapid, and scalable editing of microbial genomes.                                                              

  • Design of microbial cell factories for bio-based products, translating synthetic biology and evolution into sustainable bioprocesses and next-generation biorefineries.

           

We apply genomics and experimental evolution to Saccharomyces cerevisiae, Escherichia coli, and other microbes to uncover the genetic changes that drive adaptation to industrial stresses. Using the EasyGuide CRISPR toolkit and in vivo multipart DNA assembly, we perform precise, rapid genome edits to test genotype–phenotype relationships. Our aim is to understand evolution at the molecular level and to build robust microbial cell factories for sustainable, bio-based products. Our lab is located in Rio Claro at the Bioenergy Research Institute (IPBEN) of São Paulo State University (UNESP).

Projects

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ALE for yeast tolerance to lignocellulosic hydrolysates

(Saccharomyces cerevisiae)



New advances in adaptive evolution protocols, QTL mapping, and CRISPR/Cas9 technologies are proposed to enhance yeast tolerance to lignocellulosic hydrolysates. Learn more...

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EasyGuide CRISPR  (S. cerevisiae and E. coli)

Leveraging in vivo cloning in yeast and E. coli to assemble gRNA and donor cassettes for multilocus edits, CRISPRi, CRISPRa, and base editors. EasyAssembler: rapid in vivo assembly of multipart DNA.

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ReMaSSing - Reiterated Mass Selection and backcrosSing (S. cerevisiae)

A versatile platform for strain development that supports guided breeding, QTL mapping, genome debugging, and adaptive genotype selection.

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Adaptive evolution of Escherichia coli for  sucrose consumption

We also conduct evolutionary experiments with E. coli, which we intend to use as a workhorse for the production of polyhydroxyalkanoates from sugarcane 

sucrose.

Experimental evolution of ethanol tolerance

(Saccharomyces cerevisiae PE-2)



Here we use the S. cerevisiae strain PE-2, widely used in the Brazilian bioethanol industry, to challenge cellular adaptation to an increasing ethanol concentration.

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Support

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Genome Engineering and Evolution of Microorganisms

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