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EasyGuide CRISPR Suite:

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• A modular and versatile platform for genome editing and synthetic biology in E. coli and S. cerevisiae.                                                           

• In vivo cloning of gRNAs — Seamless assembly of single or multiple guide RNAs directly in E. coli and S. cerevisiae using the pEasyG1–G4 plasmid series.                                                                                                             

• Multiplex genome editing — Simultaneous targeting of multiple loci for rapid construction of complex genotypes.                                                                                                              

• EasyAssembler — A companion system for multipart DNA assembly, enabling combinatorial donor engineering and pathway construction.                                                                         

• EasyOligo method — Uses PCR amplification of oligonucleotides to generate gRNA and donor cassettes, eliminating traditional cloning steps.                                                                                                                              

• Genome-wide programmable regulation — In vivo cloning of large gRNA libraries to drive CRISPR interference (CRISPRi), activation (CRISPRa), and cytidine deaminase base editing across the genome.

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